Abstract
When performing spatial or temporal laser speckle contrast imaging (LSCI), contrast is generally estimated from localized windows containing limited numbers of independent speckle grains NS. This leads to a systematic bias in the estimated speckle contrast. We describe an approach to determine NS and largely correct for this bias, enabling a more accurate estimation of the speckle decorrelation time without recourse to numerical fitting of data. Validation experiments are presented where measurements are ergodic or non-ergodic, including in vivo imaging of mouse brain.
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