Abstract
The coronin family of actin‐binding proteins consisting of seven proteins in mammals is known to play a critical role in various actin‐driven processes. Intestinal epithelial cells (IECs) abundantly express a member of coronin protein family, Coronin 1C. Since actin cytoskeletal restructuring is required for cell migration observed during pathologic processes, such as wound closure, we investigated the role of Coronin 1C in the regulation of IEC cell migration. Migration of a model intestinal epithelial cell line SK‐CO15 on different extracellular matrixes and cell invasion into a 3 dimensional (3D) matrix was determined. siRNA‐mediated knock‐down of Coronin 1C enhanced the formation of membrane protrusions in cells grown in 3D culture and increased their invasiveness into 3D matrix. Furthermore, the depletion of Coronin 1C significantly increased the rate of actin‐based motility in wounded SK‐CO15 cells by increasing the persistence of membrane protrusion at the leading edge of migrating cells. These findings support a negative regulatory role for Coronin 1C in IEC migration.This research is supported by the Crohn's and Colitis Foundation of America (for SNS) and NIH (DK55679 for AN).
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