Abstract
Coronavirus Antibodies in Bat Biologists
Highlights
Of 350 registered biologists, 90 (26%) participated
The serum samples from all 90 participants were negative for antibodies against inactivated SARSCoV, and samples from all but 1 were negative for severe acute respiratory syndrome (SARS)-CoV N protein
Because the N protein has a region that is relatively conserved among all known coronaviruses [7], the antibodies against SARS-CoV N protein could have been induced by other CoVs
Summary
Of 350 registered biologists, 90 (26%) participated. Of participants, 89% had worked with or studied bats in North America, 21% in South America, 11% in Africa, 8% in Asia, 7% in Europe, and 6% in Australia. The serum samples from all 90 participants were negative for antibodies against inactivated SARSCoV, and samples from all but 1 were negative for SARS-CoV N protein. The 1 positive sample gave a strong signal (optical density 1.08 at 405 nm at a 1:400 dilution) by SARS-CoV N protein EIA and against SARS-CoV N by Western blot but gave no reactivity against recombinant SARSCoV spike protein or inactivated SARS-CoV by either EIA or Western blot.
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