Abstract
Purpose: To evaluate the changes in cornea in Sjögren’s syndrome (SS) with a novel confocal microscopy device. Methods: Twenty-three right eyes of patients with SS (23 women; mean age, 65.4 ± 11.4 years) and 13 right eyes of 13 age- and sex-matched control subjects (13 women; mean age, 68.8 ± 9.8 years) were studied. Furthermore, eight right eyes of patients with SS (8 women; mean age, 66.9 ± 9.6 years) were studied to evaluate the corneal microscopic alterations after the treatment with topical 3% diquafosol sodium eye drops. All cases had tear quantity, tear breakup time (BUT), ocular surface staining measurements, and corneal in vivo laser-scanning confocal microscopy examinations. The density and area of corneal epithelial cells (superficial, wing, and basal), density of corneal stromal cells (anterior, intermediate, and posterior), density and area of corneal endothelial cells, density and morphology of corneal sub-basal nerve plexus, density of corneal sub-basal inflammatory cells were also assessed. Results: The tear quantity, stability, and vital staining scores were significantly worse in patients with SS than in control subjects (p < 0.0001). Corneal superficial epithelial cell density was significantly lower in SS compared with control subjects (p < 0.0001). Corneal superficial epithelial cell area was significantly larger in SS compared with control subjects (p = 0.007). Corneal sub-basal nerve fiber density was lower in SS compared with control subjects (p < 0.0001). Morphological abnormality of nerve fibers was observed in SS patients. Corneal sub-basal inflammatory cell density was significantly higher in SS patients compared with control subjects (p < 0.0001). Furthermore, the mean corneal superficial epithelial cell density and area, inflammatory cell density, corneal sub-basal nerve fiber density, and morphological abnormality of nerve fibers, were improved with topical 3% diquafosol sodium treatment in the dry eye patients with SS (p < 0.05). Conclusions: The diagnostic modality using in vivo laser-scanning confocal microscopy was a useful method for the evaluation of the corneal cell density and area, nerve fiber density and morphology, and inflammatory cell density in patients with SS and also a useful tool in the assessment of treatment effect with topical 3% diquafosol sodium in the SS patients.
Highlights
The pathogenesis of Sjögren’s syndrome (SS) still remains a mystery today while genetic and environmental factors have been implicated as important [1]
In accordance with the other studies in the literature [7,8,9,10], we previously reported significantly lower tear quantity and stability values and ocular surface epithelial damage scores in patients with SS when compared with control subjects [1]
We investigated the corneal alterations in SS patients after the treatment with topical 3% diquafosol sodium as assessed by confocal microscopy
Summary
The pathogenesis of Sjögren’s syndrome (SS) still remains a mystery today while genetic and environmental factors have been implicated as important [1]. It is well-known that SS causes a tear-deficient dry eye, which leads to the ocular surface epithelial damage. The SS patients with ocular surface damage have various ocular symptoms such as discomfort, which have a bad effect on the quality of life. In accordance with the other studies in the literature [7,8,9,10], we previously reported significantly lower tear quantity and stability values and ocular surface epithelial damage scores in patients with SS when compared with control subjects [1]. It has been difficult to study corneal microscopic alterations including the epithelial damage at the cellular level since methods such as impression cytology can be invasive [11]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
