Abstract
A novel core-shell DNA self-assembly catalyzed by thiol-disulfide exchange reactions was proposed, which could realize GSH-initiated hybridization chain reaction (HCR) for signal amplification and molecules gathering. Significantly, these self-assembled products via electrostatic interaction could accumulate into prominent and clustered fluorescence-bright spots in single cancer cells for reduced glutathione monitoring, which will effectively drive cell monitoring into a new era.
Highlights
Inspired by that mentioned above, we proposed a core-shell DNA self-assembly catalyzed via thiol-disulfide exchange reactions that could realize target-initiated hybridization chain reaction (HCR) for reduced glutathione detection
A non-destructive signal amplification tactic for GSH monitoring in living cells was developed
This core-shell self-assembly had a nuclear nanosphere and an outer layer of fluorogen-labeled nucleic acid congeries, attributed to the HCR products triggered via thiol-disulfide exchange reactions
Summary
Inspired by that mentioned above, we proposed a core-shell DNA self-assembly catalyzed via thiol-disulfide exchange reactions that could realize target-initiated hybridization chain reaction (HCR) for reduced glutathione detection. A non-destructive signal amplification tactic for GSH monitoring in living cells was developed This core-shell self-assembly had a nuclear nanosphere and an outer layer of fluorogen-labeled nucleic acid congeries, attributed to the HCR products triggered via thiol-disulfide exchange reactions. These HCR products had a regular conformation, self-assembled on the surface of cores via electrostatic interaction, as core-congeries It could produce a GSH-activity-related fluorescence signal via the fluorescence resonance energy transfer, due to the fluorophore (FAM) relatively far away from the quencher (BHQ). Because this unique DNA self-assembly resulted in core-shell nanostructure with a positively charged interlayer and nucleic acid congeries, it could offer high efficiency for cellular delivery[35] and fluorescence activation
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