Abstract
Semibatch combined suspension–emulsion polymerizations are performed for production of polymer supports to be used for immobilization of lipase B from Candida antarctica (CALB), using styrene, methyl methacrylate, and cardanol as monomers. It is shown that the obtained polymer particles present the characteristic core–shell particle structure, with specific areas and average pore sizes that are comparable to values presented by Accurel MP 1000. It is also shown that the produced particles can be used successfully for immobilization of CALB, leading to immobilization efficiencies and enzyme activities that are better than the ones obtained with Accurel MP 1000.
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