Abstract
To access hydrogen peroxide scavenging activity, we propose a sensor based on core-shell iron-nickel hexacyanoferrate nanoparticles. On the one hand, the sensor preparation procedure is simple: syringing the nanoparticles suspension with subsequent annealing. On the other hand, the sensor demonstrates a stable response to 0.05 mM of H2O2 within one hour, which is sufficient for the evaluation of antioxidant activity (AO). The analytical performance characteristics of the sensor (0.5–0.6 A M−1 cm−2, detection limit 1.5 × 10−7 M and linear dynamic range 1–1000 µM) are leads to advantages over the sensor based on Prussian Blue films. The pseudo-first-order constant of hydrogen peroxide scavenging was chosen as a characteristic value of AO. The latter for trolox (standard antioxidant) was found to be linearly dependent on its concentration, thus allowing for the evaluation of antioxidant activity in trolox equivalents. The approach was validated by analyzing real beverage samples. Both the simplicity of sensor preparation and an expressiveness of analytical procedure would obviously provide a wide use of the proposed approach in the evaluation of antioxidant activity.
Highlights
We propose sensors based on Prussian Blue (PB)-nickel hexacyanoferrate (NiHCF) nanoparticles, which are characterized by high sensitivity and operational stability combined with a rapid and simple preparation technique for measuring antioxidant activity by monitoring of hydrogen peroxide scavenging
The SEM image of the PB-NiHCF nanoparticle-modified electrodes (Figure S1c, Supplementary Materials) demonstrates the complete coating, which consists of smaller structure elements than PB film (Figure S1b, Supplementary Materials) on the carbon electrode surface (Figure S1a, Supplementary Materials)
The charge transfer resistance of the PB-NiHCF nanoparticle coating measured by electrochemical impedance spectroscopy was 5–10 Ohm·cm−2 (Figure S3, Supplementary Materials) which corresponds to the same parameter of the PB
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. The thiobarbituric acid reactive substances (TBARS) assay [17], as the most common method of this group, allows for the measurement of the ability of the antioxidant to prevent or enhance the effect of ROS on lipids. Such methods are time consuming and require the use of a lipid substrate. We propose sensors based on PB-NiHCF nanoparticles, which are characterized by high sensitivity and operational stability combined with a rapid and simple preparation technique for measuring antioxidant activity by monitoring of hydrogen peroxide scavenging
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