Abstract
Compound xueshuantong capsule (CXC) is an oral traditional Chinese herbal formula (CHF) comprised of Panax notoginseng (PN), Radix astragali (RA), Salvia miltiorrhizae (SM), and Radix scrophulariaceae (RS). The present investigation was designed to explore the core bioactive components promoting blood circulation in CXC using high-performance liquid chromatography (HPLC) and animal studies. CXC samples were prepared with different proportions of the 4 herbs according to a four-factor, nine-level uniform design. CXC samples were assessed with HPLC, which identified 21 components. For the animal experiments, rats were soaked in ice water during the time interval between two adrenaline hydrochloride injections to reduce blood circulation. We assessed whole-blood viscosity (WBV), erythrocyte aggregation and red corpuscle electrophoresis indices (EAI and RCEI, respectively), plasma viscosity (PV), maximum platelet aggregation rate (MPAR), activated partial thromboplastin time (APTT), and prothrombin time (PT). Based on the hypothesis that CXC sample effects varied with differences in components, we performed grey relational analysis (GRA), principal component analysis (PCA), ridge regression (RR), and radial basis function (RBF) to evaluate the contribution of each identified component. Our results indicate that panaxytriol, ginsenoside Rb1, angoroside C, protocatechualdehyde, ginsenoside Rd, and calycosin-7-O-β-D-glucoside are the core bioactive components, and that they might play different roles in the alleviation of circulation dysfunction. Panaxytriol and ginsenoside Rb1 had close relevance to red blood cell (RBC) aggregation, angoroside C was related to platelet aggregation, protocatechualdehyde was involved in intrinsic clotting activity, ginsenoside Rd affected RBC deformability and plasma proteins, and calycosin-7-O-β-D-glucoside influenced extrinsic clotting activity. This study indicates that angoroside C, calycosin-7-O-β-D-glucoside, panaxytriol, and protocatechualdehyde may have novel therapeutic uses.
Highlights
Traditional Chinese medicine (TCM) employs compounds called Chinese herbal formulas (CHFs)
whole-blood viscosity (WBV) reflects the intrinsic resistance of blood flow in the vasculature, and abnormal WBV is associated with increased cardiovascular risk
WBV is determined by red blood cell (RBC) and by plasma viscosity (PV), which depends on the type and concentration of plasma proteins
Summary
Traditional Chinese medicine (TCM) employs compounds called Chinese herbal formulas (CHFs). In order to better guarantee the clinical safety and effectiveness of CHF, the bioactive components evidenced by suitable methods should be controlled. Studies on the relevance between drug components and effects have provided valuable information to improve TCM development, including quality control, classification, CHF prescription analysis and optimization, the identification of new medicinal plant sources, and drug design. The concept of blood stasis is described in TCM theory as a slowing or pooling of the blood due to disruption of the heart’s Qi [9]. It is often understood in biomedical terms as hematological disorders, such as hemorrhage, congestion, thrombosis, and local ischemia [9].
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