Corallincola spongiicola sp. nov., isolated from sponge.

Abstract

A gram-negative, motile, strictly aerobic, and rod-shaped bacterium designated 176GS2-150T was isolated from the sponge Hymeniacidon sinapium. The taxonomic position of the novel isolate was confirmed using the polyphasic approach. Strain 176GS2-150T grew well at 25°C on marine agar. Based on its 16S rRNA gene sequence, we showed that strain 176GS2-150T belongs to the family Psychromonadaceae and class Gammaproteobacteria and is related to Corallincola platygyrae JLT2006T (96.84% sequence similarity). The G + C content of the genomic DNA was 49.0mol%. The assembled draft genome of strain 176GS2-150T was 4.2 Mbp and consisted of 14 contigs. The major respiratory quinone was Q-8, and the major fatty acids were summed feature 3 (comprising C16 :1ω6c and/or C16:1ω7c), summed feature 8 (comprising C18 :1ω7c and/or C18:1ω6c), C17:0 iso, C16:0, and C15:0 iso. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, 3 unidentified phospholipids, and 1 unidentified polar lipid. On the basis of the genotypic and phenotypic characteristics, strain 176GS2-150T can be placed as a new species within the genus Corallincola; the name Corallincola spongiicola sp. nov. has been proposed, with type strain 176GS2-150T (= KACC 19890T = LMG 31317T).