Coptisine Induces Apoptosis in Human Hepatoma Cells Through Activating 67-kDa Laminin Receptor/cGMP Signaling.

Li Zhou,Yali Liu,Guobing Li,Fan Yang,Changpeng Hu,Jingbin Huang,Rong Zhang,Qian Zhang,Qin Tang

doi:10.3389/fphar.2018.00517

Abstract

Hepatocellular carcinoma (HCC) is the most common primary cancer of the liver. Hence, new anti-liver cancer treatment strategies need to be urgently developed. Coptisine is a natural alkaloid extracted from rhizoma coptidis which exhibits anticancer activity in various preclinical models, including liver cancer. However, the molecular mechanisms underlying the anti-liver cancer effects of coptisine remains unclear. We used flow cytometry to assess the binding of coptisine to 67LR expressed on the surface of SMMC7721, HepG2, LO2 and H9 cells. Then SMMC7721, HepG2 and BEL7402 cells, belonging to the HCC cell lines, were treated with coptisine. The cell viability was detected using a cell counting kit-8 assay. Apoptosis was evaluated using flow cytometry and transferase-mediated dUTP nick-end labeling (TUNEL) assay. Apoptotic-related proteins and tumor death receptor 67-kDa laminin receptor (67LR) were detected using Western blot analysis. The cyclic guanosine 3′,5′-monophosphate (cGMP) concentration was determined using enzyme-linked immunosorbent assay. sh67LR lentivirus, anti67LR antibody, and cGMP inhibitor NS2028 were used to determine how a 67LR/cGMP signaling pathway regulated coptisine-induced apoptosis. Tumor growth inhibited by coptisine was confirmed in a SMMC7721 cell xenograft mouse model. Coptisine selectively exhibited cell viability in human hepatoma cells but not in normal human hepatocyte cell line LO2 cells. Coptisine promoted SMMC7721 and HepG2 cell apoptosis by increasing 67LR activity. Both 67LR antibody and sh67LR treatment blocked coptisine-induced apoptosis and inhibition of cell viability. Coptisine upregulated the expression of cGMP. Moreover, cGMP inhibitor NS2028 significantly decreased coptisine-induced apoptosis and inhibition of cell viability. In vivo experiments confirmed that coptisine could significantly suppress the tumor growth and induce apoptosis in SMMC7721 xenografts through a 67LR/cGMP pathway. Coptisine-mediated 67LR activation may be a new therapeutic strategy for treating hepatic malignancy.

Highlights

Hepatocellular carcinoma (HCC), one of the most common malignancies worldwide, comprises only 10–30% surgical candidates (Gravitz, 2014; Jin et al, 2017)
Coptisine treatment induced relatively lower toxicity in LO2 cells than in SMMC7721 cells (Figures 1B,D,F). These results suggested that coptisine selectively inhibited cell viability in human HCC SMMC7721, HepG2 and BEL7402 cells but not in normal human hepatocyte cell line LO2 cells
Our results demonstrated that coptisine selectively induced apoptosis in SMMC7721, HepG2 and BEL7402 cells (HCC cell lines) and inhibited the tumor growth in xenografts without obvious toxicity, suggesting that coptisine could be developed into a novel anti-tumor agent for treating liver cancer

Summary

Introduction

Hepatocellular carcinoma (HCC), one of the most common malignancies worldwide, comprises only 10–30% surgical candidates (Gravitz, 2014; Jin et al, 2017). Chemotherapy and some oral multikinase inhibitors are primarily used to treat intermediate–advanced HCC (Zhang et al, 2016). More effective pharmacological agents for treating HCC are urgently needed. An isoquinoline alkaloid, is extracted from rhizoma coptidis. It has a variety of pharmacological effects, such as antibacterial (Kwon et al, 2008), anticachectic (Iizuka et al, 2002; Iizuka, 2016), and lipid-lowering (Kou et al, 2016). Another study (Rao et al, 2017) found that coptisine could significantly induce mitochondria-mediated apoptosis in non-small-cell lung cancer A549 cells. The present study indicated that coptisine selectively inhibited cell viability in human HCC cell line SMMC7721, HepG2 and BEL7402 cells but not in normal human hepatocyte cell line LO2 cells, suggesting that coptisine might be a potential cancer treatment for HCC. Its mechanism of action still need further research (Lin et al, 2004; Huang et al, 2015)

Methods

Results

Discussion

Conclusion