Abstract
The objectives of this study were to determine the effects of hydrogenated vegetable oil, cocoa butter, fresh and cured pork from high (spinach = 0.2%) and low (spinach = 0.02%) nitrate/nitrite containing diets on copper status of mice. The dietary treatments had no significant effects on urinary and fecal copper excretions and whole blood and liver copper contents of mice. However, there were significant (p < 0.01) interactions of protein source by type of fat and protein source by spinach (nitrate/nitrite) level with regard to fecal copper excretion. Feeding of cocoa butter tended to result in lower copper excretions in urine and feces and higher copper concentrations in the whole blood and liver of mice. Therefore, cocoa butter might enhance copper absorption in comparison to hydrogenated vegetable oil.
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