Abstract
Copper oxide nanosheets (CuO NSs) have been successfully obtained by exploiting an effective one-step approach of sugar-blowing method followed by calcination. The nanosheets were characterized by several techniques like X-ray powder diffraction (XRD), Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). Impressively, CuO NSs display haloperoxidase (HPO) like catalytic activity which catalyses the oxidation of chloride ions by H2O2 giving rise to reactive chlorine species (RCS). A sensitive and selective colorimetric sensor was then demonstrated via the oxidation of chromogenic substrate 3,3′,5,5′- tetramethylbenzidine (TMB) by the novel nanoenzyme CuO NSs through the generation of RCS for H2O2 and glucose detection with limit of detection of 109 nM and 21 nM in the linear ranges of 4.6 µM to 769 µM and 0.22 µM to 19.57 µM respectively. Additionally, the methodology is validated for the analysis of glucose in real samples.
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