Copper-induced changes in locomotor behaviors and neuronal physiology of the freshwater oligochaete, Lumbriculus variegatus

Abstract

The behavioral and neurotoxic effects of copper exposure were examined in the freshwater oligochaete, Lumbriculus variegatus. The 24 h LC 50 for worms exposed to copper sulfate in an artificial pond water was 0.45 μM. Almost all animals that died due to copper exposure died during the first day of exposure. Immersion in water containing 0.2 or 0.4 μM copper produced time- and concentration-dependent reductions in the ability of tactile stimulation to evoke two stereotyped locomotory behaviors, body reversal and helical swimming. Helical swimming was more severely affected by copper exposure than was body reversal behavior. Upon return to clean water, both behaviors returned to normal levels within 1–2 days. Noninvasive electrophysiological testing indicated that copper exposure produced time- and concentration-dependent reductions in the conduction velocities of the medial and lateral giant nerve fibers. An 8 h exposure to 0.2 μM copper produced significant reductions in giant fiber conduction velocities that returned to normal levels within 3 days of return to clean water. It is likely that copper exposure can significantly degrade the ability of aquatic oligochaetes to avoid predators.