Abstract
The study delineates the development and characterization of carboxymethyl chitosan–gelatin-copper oxide (CuO) crosslinked scaffolds for biomedical applications. Four scaffolds were developed using lyophilization technique using two stage crosslinking and evaluated using FT-IR, TGA to ascertain the crosslinking. Further, swelling index (SI), hydrolytic degradation, water vapour transmission rate (WVTR) as well as morphological characterization of scaffolds were carried out using SEM and optical profilometer. Additionally, modified disc diffusion method and MTT assay was carried out to check antibacterial action and cyto-compatibility. The results revealed that SI, hydrolytic degradation, and WVTR changes with the change in percentage of CuO. Further, effect of CuO concentration was evident from roughness variations of the developed scaffolds. Additionally SEM images shows presence of micro pores and the scaffolds exhibited good antibacterial action both against Gram positive and Gram negative bacteria. Lastly cytotoxicity assay exhibited good cell viability even at higher concentration of CuO nanoparticles. Thus, this work represents a facile approach to develop CuO crosslinked scaffolds of CMCh-gelatin that may potentially be explored further for biomedical applications.
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