Copper‐ Based Metal‐Organic Frameworks as Peroxidase Mimics Leading to Sensitive H2O2 and Glucose Detection

Abstract

AbstractA copper based metal organic framework (MOF) synthesized under solvo‐thermal conditions was found to exhibit intrinsic peroxidase‐like activity and was established both spectrophotometrically and electrochemically. It was found that the catalytic behaviour had been consistent with Michaelis‐Menten kinetics with a Km value of 0.08 mM with H2O2, relatively lower than that of Horseradish peroxidase enzyme (HRP) ie; 3.7 mM. The suitability of Cu–MOF for sensing H2O2 was investigated by electrochemical means. In the case of electrochemical studies, the Cu–MOF modified GCE (glassy carbon electrode) showed a very high sensitivity of 263 μA mmol−1 cm−2 with a detection limit up to 35 mM for H2O2. The peroxidase mimics can be combined with the enzymatic oxidation of glucose for sensing glucose. The detection of H2O2 and glucose using Cu–MOF was found to be highly selective towards the common interfering species.