Abstract
The freshwater chrysophyte Synura sp. was grown in batch cultures up to stationary growth phase, harvested, and the dissolved organic material collected. Gas–liquid chromatographic analysis showed that mannose and xylose made up 50% of the total polysaccharide composition. Large amounts of protein were detected in the extracellular material by spectrophotometric assay. The complexation properties (conditional stability constant and total ligand concentration) presented by the polysaccharide were investigated using copper and lead ion selective electrodes for complexometric titrations. Scatchard plot analysis revealed that Synura sp. produces one major ligand characterized by high affinity for copper and moderate affinity for lead and another ligand with very low affinity for either metal.
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