Abstract
ObjectivesColorectal cancer (CRC) is one of the most common malignant human tumors. It is associated with high morbidity and mortality rates. In recent years, tumor gene therapy has emerged as a promising new approach for colorectal cancer therapy. Herein, we identify and analyze the role of COPB2 (coatomer protein complex, subunit beta 2) in proliferation and apoptosis of CRC cells.MethodsTo investigate the role of COPB2 in the proliferation and apoptosis of CRC cells, a shCOPB2 vector and a shCtrl vector were constructed for transfection into RKO and HCT116 cells. Cells proliferation was subsequently measured via cell counting kit-8 (CCK8) assay and Celigo cell counting assay. Apoptosis was measured via flow cytometry. The activity level of Caspase 3/7 was measured. Finally, the level of several JNK/c-Jun apoptosis pathway-related proteins were measured to characterize the mechanism of apoptosis.ResultsOur results showed that the proliferation rate was decreased and the apoptosis rate was increased in shCOPB2-treated RKO and HCT116 cells compared to those in controls. After the silencing of COPB2, JNK/c-Jun signal pathway activation was increased, the expression levels of apoptosis pathway-related proteins, such as Bad, p53 and Caspase 3, were also increased.ConclusionCOPB2 gene silencing can inhibit RKO and HCT116 cells proliferation and induce apoptosis via the JNK/c-Jun signaling pathway.
Highlights
Colorectal cancer (CRC) is the most common malignant tumor of the human digestive system [1]
Our results showed that the proliferation rate was decreased and the apoptosis rate was increased in shCOPB2-treated RKO and HCT116 cells compared to those in controls
This study reveals that COPB2 is essential for the proliferation and apoptosis of colon cancer indicating that it may serve as a potential target for the treatment of CRC
Summary
CRC is the most common malignant tumor of the human digestive system [1]. CRC accounts for approximately 8% of all cancer deaths [4] and is the third-leading cause of death from cancer among adults around the world [5,6]. High incidence and mortality rates due to CRC have been identified in China. Treatment for CRC primarily based on surgery [9,10], but often includes chemotherapy [11], radiotherapy [12], and biotherapy, and other therapies [13,14]. With advancements in genetic engineering technology, gene therapy has attracted increasing attention as a novel method of CRC treatment [15,16,17]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
