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Abstract
Ultraviolet (UV) exposure is well-known to induce premature aging, which is mediated by matrix metalloproteinase-1 (MMP-1) activity. A 9-mer peptide, CopA3 (CopA3) was synthesized from a natural peptide, coprisin, which is isolated from the dung beetle Copris tripartitus. As part of our continuing search for novel bioactive natural products, CopA3 was investigated for its in vitro anti-skin photoaging activity. UV-induced inhibition of type-I procollagen and induction of MMP-1 were partially prevented in human skin fibroblasts by CopA3 peptide in a dose-dependent manner. At a concentration of 25 μM, CopA3 nearly completely inhibited MMP-1 expression. These results suggest that CopA3, an insect peptide, is a potential candidate for the prevention and treatment of skin aging.
Highlights
When skin is frequently exposed to ultraviolet (UV) irradiation, which leads to photoaging
This evidence suggests that CopA3 may act as a potent inhibitor of matrix metalloproteinase-1 (MMP-1) when fibroblasts were affected by UVB irradiation, so in this study, we investigated the effect of the insect-derived peptide CopA3 on the expression of matrix metalloproteinases (MMPs)-1 and type-I
Treatment of CCD-986sk with CopA3 at 5, 10 and 25 μg/mL caused a decrease in these levels by about 60% (Figure 2B). These results demonstrate that CopA3 peptide increased the production of type-I procollagen and decreased the production of MMP-1 in UVB-induced fibroblast cells, suggesting that CopA3 may stimulate expression of type-I procollagen and/or inhibit MMP-1 gene expression in UVB-induced fibroblast cells
Summary
When skin is frequently exposed to ultraviolet (UV) irradiation, which leads to photoaging. The photoaging process results in major skin alterations through stimulation of multiple signal transduction pathways, which lead to activation of transcription factors or target genes [1]. A recent study has demonstrated that retinoic acid prevents photoaging from UV irradiation by altering multiple signal transduction pathways [1]. Taken together, this evidence suggests that CopA3 may act as a potent inhibitor of MMP-1 when fibroblasts were affected by UVB irradiation, so in this study, we investigated the effect of the insect-derived peptide CopA3 on the expression of MMP-1 and type-I procollagen in UVB-induced CCD-986sk cells
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