Abstract
The tautomeric equilibrium in the H-bonded pair guanine-cytosine was studied by the semiempirical MNDO/HB quantum-chemical method. It was shown that hydration of the bases leads to an appreciable reduction of the activation barrier in the synchronous exchange of protons between the bases. Desolvation of the active center of the polymerase reduces the probability of error in the synthesis of the complementary chain of the nucleic acid by ∼106 times. Deprotonation of the phosphate groups of the nucleotides destabilizes the transition state in proton exchange. The genetic information is conserved as a result of the formation of nucleoprotein complexes with an ionic bond between the phosphate residue of the saccharophosphate skeleton of the nucleic acid and an amino (imino) group of the protein.
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Schedule a callMore From: Bulletin of the Academy of Sciences of the USSR Division of Chemical Science
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