Abstract
The miR-23a~27a~24-2 cluster is an important regulator in cell metabolism. However, the cooperative and independent functions of this cluster in bovine adipocyte adipogenesis have not been elucidated. In this study, we found that expression of the miR-23a~27a~24-2 cluster was induced during adipogenesis and this cluster acted as a negative regulator of adipogenesis. miR-27a and miR-24-2 were shown to inhibit adipogenesis by directly targeting glycerol-3-phosphate acyltransferase, mitochondrial (GPAM) and diacylglycerol O-acyltransferase 2 (DGAT2), both of which promoted adipogenesis. Meanwhile, miR-23a and miR-24-2 were shown to target decorin (DCN), glucose-6-phosphate dehydrogenase (G6PD), and lipoprotein lipase (LPL), all of which repressed adipogenesis in this study. Thus, the miR-23a~27a~24-2 cluster exhibits a non-canonical regulatory role in bovine adipocyte adipogenesis. To determine how the miR-23a~27a~24-2 cluster inhibits adipogenesis while targeting anti-adipogenic genes, we identified another target gene, fibroblast growth factor 11 (FGF11), a positive regulator of adipogenesis, that was commonly targeted by the entire miR-23a~27a~24-2 cluster. Our findings suggest that the miR-23a~27a~24-2 cluster fine-tunes the regulation of adipogenesis by targeting two types of genes with pro- or anti-adipogenic effects. This balanced regulatory role of miR-23a~27a~24-2 cluster finally repressed adipogenesis.
Highlights
Fat deposition is highly correlated with beef carcass grading and characteristics that contribute to meat quality such as juiciness, tenderness, and flavor
To establish a system to study the effects of miRNAs on the adipogenic differentiation of bovine preadipocytes, the adipogenic potential of preadipocytes isolated from perirenal adipose tissue was evaluated
The total cytoplasm triglyceride (TG) content was significantly repressed by the miRNA agomir (Figure 2E). These results indicate that the miR-23a~27a~24-2 cluster is a negative regulator of bovine preadipocyte differentiation
Summary
Fat deposition is highly correlated with beef carcass grading and characteristics that contribute to meat quality such as juiciness, tenderness, and flavor. It is essential to advance our understanding of the molecular mechanisms that affect fat deposition so that genomic selection can be used to produce high-grade beef with consistent quality. Adipogenesis of progenitor cells into mature adipocytes is tightly controlled by various factors [2,3]. Among these factors, miRNAs have been reported to have a close relationship with fat deposits and meat quality [4,5]. The exact roles of many miRNAs in adipogenesis have not yet been determined
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