Cooling ameliorates decreased milk protein metrics in heat-stressed lactating Holstein cows

Abstract

Cooling can alleviate the negative consequences of heat stress on multiple milk production metrics in dairy cows. However, it is still controversial whether cooling can increase milk protein content compared with heat-stressed cows. The objective of the present study was to evaluate the relief effect of cooling on the decrease in milk protein concentration during heat stress and elucidate the potential metabolic mechanisms. Thirty lactating multiparous Holstein cows (days in milk = 175 ± 25 d, milk yield = 27.5 ± 2.5 kg/d; mean ± SD) were assigned to 1 of 3 treatments: heat stress (HS; n = 10), cooling (CL; n = 10), and cooling with pair-feeding (PFCL; n = 10). The barns for PFCL and CL cows were equipped with sprinklers and fans, whereas the barn for HS cows were not. The average temperature-humidity index during the experiment ranged from 74 to 83. The spraying was activated automatically 2 times per day (1130-1330 h and 1500-1600 h) with 3 min on and 6 min off during the first 2 wk, and 1.5 min on and 3 min off during the last 2 wk, whereas the fans operated 24 h/d. The experiment lasted for 4 wk in total. Milk, urine, feces, total mixed ration, blood, and rumen fluid samples were collected weekly. Compared with HS, feed efficiency (1.24 and 1.49), milk protein yield (0.82 and 0.94 kg/d), and milk fat yield (0.98 and 1.26 kg/d) were increased in PFCL, whereas the differences between CL and HS were not significant. Compared with HS cows, PFCL and CL cows had a lower respiratory rate (70.6, 59.1, and 60.3 breaths per minute, respectively), rectal temperature (38.95, 38.61, and 38.51°C), and shoulder skin temperature (33.95, 33.25, 33.40°C), and had greater milk protein content (3.41, 3.72, and 3.69%) and milk fat percent (4.08, 4.97, 4.65%). Both the blood activity of catalase (increased by 12.8 and 41.0%) and glutathione peroxidase (12.6 and 40.4%) of PFCL and CL cows were greater than the HS cows. Compared with HS, cooling increased the blood content of glucose, methionine, threonine, and cystathionine by 10.7% and 10.3%, 19.0% and 9.5%, 15.8% and 12.0%, and 9.5% and 23.8% in PFCL and CL, respectively. In conclusion, the results indicated that cooling partially rescued milk protein synthesis induced by heat stress, and the potential mechanism may have been due to increased antioxidant ability, blood glucose, and key AA. Consequently, in addition to modifying the environment, nutritional and physiological strategies designed to influence carbohydrate, AA, and oxidative homeostasis may be an opportunity to maintain or correct low milk protein content during the warm summer months.