Conversion of VP1 to VPg in Cells Infected by Infectious Pancreatic Necrosis Virus

Abstract

The RNA polymerase VP1 of IPNV (a bisegmented dsRNA containing virus) is present in the virion both as a free polypeptide and as a genome-linked protein (VPg). Virion VP1 primes viral RNA synthesisin vitro(P. Dobos, 1995,Virology208, 19–25), and here we present data which suggest that protein-primed RNA synthesis may also take place in infectious pancreatic necrosis virus (IPNV)-infected cells. Anti-VP1 serum immunoprecipitated several polypeptides larger than the 94-kDa VP1 of IPNV from [35S]methionine-labeled infected cell lysates. During denaturing, SDS–polyacrylamide gel electrophoresis these polypeptides formed a characteristic “ladder” which was resistant to alkaline phosphatase but sensitive to RNases, indicating that it consisted of VP1 polypeptides with oligoribonucleotides of various lengths attached to them. Probing the ladder with 5′ and 3′ end-specific, as well as plus-, or minus-strand-specific oligonucleotides revealed that they represent VP1 linked to 5′ terminal sequences of genome segment A- and B-specific plus strands. Pulse–chase experiments in combination with two-dimensional polyacrylamide gel electrophoresis revealed that labeled VP1 could be chased to replicative intermediate, to ssRNA, to dsRNA, and eventually to virion VPg-dsRNA and that VP1 could be released from all these structures by RNase treatment. We suggest that these results are most compatible with the model where a VP1-pN structure acts as a primer for viral RNA synthesisin vivo,a mechanism that has been shown to occurin vitro.