Conversion of Sugar Cane Bagasse Become Bioethanol Using Variations of Yeast Types and Fermentation Temperatures with Socarification Process

Abstract

Sugarcane bagasse is a waste of sugar factories and one of its lignocellulosic materials is still of limited use. Sugarcane bagasse consists of three main components, namely cellulose, hemicellulose, and lignin. Sugarcane bagasse can be converted into bioethanol through a hydrolysis process (the process of converting carbohydrates into glucose) which consists of a liquefaction and saccharification process and followed by a fermentation process. The hydrolysis method is carried out enzymatically. This research used alpha amylase and gluco amylase enzymes with various types of yeast including Saccharomyces cereviceae, Rhizopus oryzae, Acetobacter xylinum, Mucor sp., and Aspergilus niger varied with liquefaction temperatures including 80, 85, 90, 95, and 100 °C. Obtained the most optimal yeast is Saccharomyces cereviceae with an optimal temperature of 95 °C resulting in a bioethanol concentration of 4.5%.