Abstract
Fishery processing by-products have been of great interest to researchers due to their beneficial applications in many fields. In this study, five types of marine by-products, including demineralized crab shell, demineralized shrimp shell, shrimp head, shrimp shell, and squid pen, provided sources of carbon and nitrogen nutrition by producing a protease from Paenibacillus sp. TKU047. Strain TKU047 demonstrated the highest protease productivity (2.98 U/mL) when cultured for two days on a medium containing 0.5% of shrimp head powder (SHP). The mass of TKU047 protease was determined to be 32 kDa (approximately). TKU047 protease displayed optimal activity at 70–80 °C and pH 9, with a pH range of stability from 6 to 11. TKU047 protease also showed stability in solutions containing surfactants and detergents. Based on its excellent properties, Paenibacillus sp. TKU047 protease may be a feasible candidate for inclusion in laundry detergents.
Highlights
These results suggest that TKU047 protease is a thermostable enzyme
Paenibacillus has several strains with the potential to convert fishery processing by-products into various bioactive compounds [11,12,13,30,43,48,49,50,51]
Protease was produced by Paenibacillus sp
Summary
The seafood processing industry discards a large amount of by-products, including viscera, shells, heads, squid pens, fins, and bones, even though they could be recycled to produce bioactive compounds like gelatin [1,2,3,4], enzymes [4,5,6,7,8,9,10,11,12,13,14,15,16,17], chitin [8,18,19,20,21,22,23,24,25,26], chitin oligomers [7,11,12], α-glucosidase inhibitors (aGI) [27,28,29,30,31], carotenoids [32,33], and bioactive peptides [34,35,36,37,38,39,40]. Much research has gone into converting these by-products into bioactive products that have potential applications in biotechnological, agricultural, nutritional, pharmaceutical, and biomedical industries [1,4,5,17]. Marine chitinous byproducts, such as shells of crabs and shrimps, and pens (gladius) of squids, are a great source of chitin. These materials contain a significant amount of mineral salts and proteins [4,8,17]. With a more environmentally friendly production process, these marine byproducts could be used as carbon/nitrogen sources for microorganism bioconversion
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
