Abstract
AbstractA new microbial isolate,Flavobacterium sp. strain DS5, converts linoleic acid into 10‐hydroxy‐12(Z)‐octadecenoic acid (10‐HOA) with 55% yield. The product was characterized by gas chromatography (GC), GC/mass spectrometry, nuclear magnetic resonance and Fourier transform infrared spectroscopy. The specific optical rotation of 10‐HOA is [α]D24=−5.58 (methanol). The optimum time, pH and temperature for the production of 10‐HOA were 36h, 7.5 and 20–35°C, respectively. The enzyme(s) that converts linoleic acid to 10‐HOA is soluble and located intracellulary in strain DS5. Two minor products, 10‐methoxy‐12‐octade‐cenoic acid and 10‐keto‐12‐octadecenoic acid, were also identified. 10‐HOA was further metabolized by strain DS5. Among the unsaturated fatty acids studied, the order of reactivity for the DS5 enzyme(s) is oleic>palmitoleic> linoleic>linolenic>γ‐linolenic>myristoleic acid.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
