Abstract
CONVERSION OF d-XYLOSE TO d-XYLULOSE IN EXTRACTS OF LACTOBACILLUS PENTOSUS
Highlights
As reported previously (I), there was no decrease in total orcinol-reacting material when the various pentoses were incubated with extracts of L. pentosus in the absence of ATP; a new substance which was resistant to oxidation by bromine was detected when xylose was the substrate (Table I)
The experiments presented here demonstrate that an enzyme catalyzing the conversion of n-xylose to n-xylulose is present in cells of L. pentosus grown on xylose but not in cells grown on glucose
Xylulose is fermented by intact cells at rates essentially identical with those obtained with similar concentrations of xylose
Summary
Evidence has been presented that D-xylose (and probably L-arabinose) is converted by Lactobacillus pentosus to esters with the ribose (or ribulose) configuration before cleavage of the carbon chain occurs (1). In experiments with extracts of L. psntosus which do not degrade ribose phosphates (a), it has recently been possible to demonstrate the following reaction.’. The data reported in t,he present communication demonstrate that extracts of xylose-adapted cells of L. pentosus catalyze the reaction. If ATP is present, xylulose is oonvert8edto a mixture of pentose phosphates similar to that obtained from xylose. Since xylulose is phosphorylated in these extracts more rapidly than is xylose, it is possiblethat xylulose is an intermediat,e in the conversion of xylose to a ribose (or ribulose) phosphate. The excess bromine was removed by gassing with nitrogen, and the samples filtered and diluted for assay of residual pentose
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