Abstract
The enzymatic conversion of d-xylose into xylitol by the immobilized cells of Candida pelliculosa (NADP + dependent xylose reductase) coupled with the immobilized cells of Methanobacterium sp. HU (hydrogenase and F 120-NADP + oxidoreductase) was done using hydrogen as an electron donor of NADP +. Benzene treatment of the co-immobilized cells with a photo-crosslinkable resin prepolymer, ENT 4000, increased the permeability of NADP (H) into the cells. Besides, treatments with glutaraldehyde and hexamethylenediamine to the immubilized cells were done to enhance the stability of immobilized-cell activity. Thus, the continuous production of xylitol in a column reactor packed with the co-immobilized cells could operate stably for 2 weeks.
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