Abstract
Artificial cells containing malate dehydrogenase (EC 1.1.1.37), leucine dehydrogenase (EC 1.4.1.9), urease (EC 3.5.1.5), and dextran-NAD + were prepared. This immobilized multienzyme system effectively converted ammonia or urea into essential amino acids, L-leucine, L-valine and L-isoleucine. L-malate acted as a cosubstrate for the regeneration of dextran-NADH. Greater concentrations of L-malate favored higher conversion ratios. The effects of ammonium salts and urea on the reaction rate were also studied. The reaction rates in ammonium salt solutions were 36.9–78.2% of those in urea solutions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
