Abstract
The present study focuses on the measurement of metabolic heat profile for the proteolytic activity by the Aspergillus tamarii. Here, agricultural by-products like wheat bran, cottonseed meal, soya flour and skimmed milk were utilized to maximize the enzyme expression. The study revealed that the maximum heat release (69.5 kJ/L) was dominantly from the oxidative breakdown of the nitrogen source i.e. the cottonseed. A distinguishable growth and non-growth enzyme expression phases of A. tamarii could be identified from the metabolic heat profiles. A maximum enzyme expression of 1.511 U/mL was obtained with a 1% supply of nitrogen source. Metabolic heat production rates were correlated to fungal protease secretion for the first time.
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