Conversion from farmland to orchards has minor effects on nitrogen biological processes in deep loess deposits

Abstract

High nitrate (NO3--N) accumulation has been observed globally in vadose zones due to the overuse of synthetic fertilizer, but its impacts on nitrogen (N) biological processes remain poorly understood, especially after land use conversion from farmlands to deep-rooted apple orchards. In this study, we collected paired soil samples to determine available N contents, NO3--N isotopes, and abundances of N functional genes (NFGs) in 10-m loess profiles under cultivated farmlands and adjacent apple orchards (n = 300). The average NO3--N accumulation under orchards was 4773 kg N ha−1 (10 times higher than farmlands), with the NO3--N profiles resembling a parabolic curve within 0 – 4 m below the surface. Regardless of excessive fertilizer application and well-developed root systems, the deep-rooted orchards had minor effects on N sources and biological processes in deep loess deposits, as indicated by our estimates of isotopes and NFGs abundances. We hypothesized that the limited and inactive microorganisms in the subsoil are more resistant to environmental disturbances, and that carbon source and ammonium substrate dominated the gene expression and occurrence of NFGs-indicated biological processes. The limited root biomass per unit depth and the inhibition effects of a dry soil horizon on root turnover and exudate release likely contributed to the less perturbed N biological processes under orchards. As the first study to explore N transformation processes in 10-m loess deposits, this study provided insights for complicated N biogeochemical cycles in arid regions with deep unsaturated soils and intensive agricultural land use.