Converging flow chromatography in constant-pressure mode

Abstract

Dispersion in chromatographic processes can be reduced to a minimum using converging columns and a curved frit at the outlet. Working at constant pressure at the inlet the internal packing is increasingly compressed by the accelerated flow. Thus the packed bed is stabilized. Under these conditions the observed flux at the outlet and the power input of the pump are inversely related to the viscosity of the eluting solute. Comparing converging flow chromatography (CFC) with classical axial flow chromatography (AFC) and radial flow chromatography (RFC) the stationary phase is used more efficiently in CFC.