Abstract
Polymerase chain reaction driven by sequence specific primers has become the most widely used diagnostic method to detect and identify plant pathogens. The sensitive and cost-effective pathogen detection is exceptionally important in the production of propagating material. In this paper we have collected primer sequence data from the literature for the detection of the most important grapevine pathogens disseminated by propagating stocks by conventional polymerase chain reaction. Basic protocols to obtain template nucleic acids have also been briefly rewieved.
Highlights
During the recent decades the polymerase chain reaction (PCR) has become the most common diagnostic protocol in plant pathology
Pathogen-free grapevine stocks can be selected from the existing plantations by various diagnostic protocols or produced by various curative (e. g., hot water) treatments and in vitro shoot tip and/or apical meristem cultures (Bisztray et al 2012)
The pathogen-free status of hot water treated and micropropagated plants should be tested by appropriate diagnostic methods
Summary
During the recent decades the polymerase chain reaction (PCR) has become the most common diagnostic protocol in plant pathology. The introduction of the conventional PCR (including multiplex, nested and reverse trascription PCR) has opened wide application possibilities due to its simplicity and cost effectiveness. Later, it was followed by the more sensitive, and more costly, quantitative realtime PCR (qRT-PCR). Quantitative real-time PCR protocols have already been developed for several viroids (Papayiannis 2014, Sun et al 2014) , viruses (Harper et al 2011, Osman & Rowhani 2008, Pacificio et al 2011), phytoplasmas
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