Abstract
Abstract The fungus Corynespora cassiicola, causal agent of target spot in soybeans, can be transmitted by soybean seeds and as of that point cause severe damage. This disease may be diagnosed at an early stage by seed testing, but knowledge in this area is insufficient. Because of that and increased attack by the disease in soybean areas in Brazil, further studies are required. The aim of this study was to evaluate the use of conventional PCR in detecting C. cassiicola in soybean seeds. The GA4-F/GA4-R primers described in the literature were tested for their specificity and sensitivity for detection of C. cassiicola in pure culture and in soybean seeds. Uninoculated and inoculated seed samples were used with different incidence levels - 100%, 10%, 1%, 0.5%, 0.25%, and 0% of preestablished inoculum potentials, P0, P1, P2, and P3. Detection of C. cassiicola in P1 inoculum potential was observed in samples with incidence levels of 10% to 100%. In the P3 potential, detection of the pathogen was successful in samples at the low level of 0.25%.
Highlights
IntroductionThe fungus Corynespora cassiicola (Berk. and M.A. Curtis) C.T
The isolates of C. cassiicola were obtained from symptomatic soybean plants; after their identity was confirmed, they were deposited in the Mycological Collection of the Seed Pathology Laboratory (Coleção Micológica do Laboratório de Patologia de Sementes - CMLAPS) of the Federal University of Lavras (UFLA), Lavras - MG, Brazil, and were stored for approximately three months (Table 1)
The DNA extracted from positive controls and negative controls were analyzed by Nano Drop 3300 (Thermo Scientific) and electrophoresis gel and exhibited sufficient quantity and purity
Summary
The fungus Corynespora cassiicola (Berk. and M.A. Curtis) C.T. The fungus is cosmopolitan and abundant in tropical regions (Kaushal, 2009) and is found in diverse soybean growing regions in Brazil This fungus is an organism that can survive in crop residue, volunteer plants and seeds, and its survival is favored by relative humidity conditions of 80% and temperatures from 20 °C to 30 °C (Henning et al, 2005; Soares et al, 2009). It is spread mainly by wind, spattering of rain (Almeida et al, 2005), and infected or contaminated seeds (Sinclair and Backman, 1989)
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