Abstract
A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/mug of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure.
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