Convenient one-pot procedure for synthesizing 4,4′-dimethoxy-boradiaza- s-indacene dyes and their application to cell labeling

Abstract

We succeeded in developing a convenient one-pot pathway for synthesizing 4,4′-dimethoxy-boradiaza- s-indacene dyes 4a– d. The structures feature two methoxy groups in place of the fluorine atoms in 4,4′-difluoro-4-boradiaza- s-indacene. These novel dyes emitted green fluorescence and possessed moderate to high fluorescence quantum yields ( Φ=0.32–0.93). We demonstrated that these dyes have applicability to cell labeling.