Abstract
Quercetin (Qu) is one of the most abundant flavonoids in the human diet. High concentrations of Qu can easily cause adverse effects and induce inflammation, joint pain and stiffness. In this study, Heme was used as a sensitive element and deposited and formed nanorods on a glassy carbon electrode (GCE) for the detection of Qu. The Heme/GCE sensor was characterized using scanning electron microscopy (SEM), cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. Under optimized conditions, the developed sensor presented a linear concentration ranging from 0.1 to 700 μmol·L−1 according to the CV and DPV methods. The detection limit for the sensor was 0.134 μmol·L−1 and its sensitivity was 0.12 μA·μM−1·cm−2, which were obtained from CV analysis. Through DPV analysis we obtained a detection limit of 0.063 μmol·L−1 and a sensitivity of 0.09 μA·μM−1·cm−2. Finally, this sensor was used to detect the Qu concentration in loquat leaf powder extract, with recovery between 98.55–102.89% and total R.S.D. lower than 3.70%. The constructed electrochemical sensor showed good anti-interference, repeatability and stability, indicating that it is also usable for the rapid detection of Qu in actual samples.
Highlights
Quercetin (Qu) is a common antioxidant, and is one of the flavonoids that the human body consumes frequently in daily meals [1,2]
Ltd. (Shanghai, China), and loquat leaf powder was purchased from market, which was directly diluted with phosphate-buffered saline (PBS) buffer for differential pulse voltammetry (DPV) experiments
electrochemical impedance spectroscopy (EIS) process techniques were used to conduct electrochemical analyses of of the sensor construction to further demonstrate that β-CD, dopamine hydrochloride (DA), Heme and Fcthe sensor further demonstrate that β-CD, DA, Hemeindicating and Fc-ECG
Summary
Quercetin (Qu) is a common antioxidant, and is one of the flavonoids that the human body consumes frequently in daily meals [1,2]. Qu mostly exists in the form of glycosides, and it can be obtained through acid hydrolysis and is widely present in the roots, stems and leaves of plants [3]. Qu shows multiple biological activities, such as anti-oxidation, anti-viral, and anti-inflammatory effects, and has demonstrated a great effect in treating prostate cancer and inhibiting the growth of isolated malignant cells [4,5]. Because of the antioxidant properties of Qu, it can remove active oxygen, hydroxyl radicals and superoxide anions very well. High concentrations of Qu tend to cause reverse effects and induce inflammation, joint pain and stiffness, and can cause abnormal changes in hormone levels in the body [6–9]. The determination of Qu content is of great significance
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