Abstract
The achievement of high hybridization efficiency in DNA microarrays is largely affected by the surface density of immobilized functional DNA probes. We investigated the chemical conditions for the formation of mixed self-assembled monolayers of thiol-modified oligonucleotides and mercaptohexanol deposited onto gold surfaces. The surface density of DNA was studied by means of high-resolution X-ray photoemission spectroscopy. The measurements revealed that the spatial density of DNA strands can be controlled within a wide range by the concentration of MgCl 2 in the immobilization buffer. Moreover, improved preparation conditions for high-quality self-assembled hybrid monolayers are presented. Compared to our previous work, a reduction in unbound thiol was achieved by changing from ethanolic to aqueous solvent and lowering the MCH concentration.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Electron Spectroscopy and Related Phenomena
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
