Controlling cisplatin release by synergistic action of silver-cisplatin on monodispersed spherical silica for targeted anticancer and antibacterial activities

Abstract

Recently, metal-based nanoformulations have been explored for targeted cancer therapeutics. In this study, synergistic therapeutic action of silver (Ag)-cisplatin nanoparticles loaded on monodispersed spherical silica (MSS) was investigated to evaluate anti-cancer (HCT-116 and HeLa cells) and antibacterial activity (P.aeruginosa, and S.aureus). Four different wt% of Ag (1, 2, 4 and 6 wt%) was loaded on MSS using a wet impregnation method. Characterization using XRD revealed the cubic phase of Ag nanoparticles with (111), (200) and (220) plane on MSS. The chemical state, dispersion of Ag and synergistic cohabitation with cisplatin (Cpt) was identified using diffuse reflectance UV–visible spectroscopy (DR-UV Vis), X-ray photoelectron spectroscopy (XPS), Scanning electron microscopy/energy-dispersive X-ray spectrometry (SEM-EDS) and High-resolution transmission electron microscopy (HR-TEM). Zeta potential analysis indicated an increase in hydrodynamic diameter with high colloidal stability (–23.3 to −28.5 mV). The percentage cumulative Cpt release was studied using automated Franz cell and dialysis membrane technique. The effect of Cpt release on 4 wt%Ag impregnated on different shaped supports was investigated using cubic SBA-16 (Santa Barbara Amorphous), MFI type Ti-ZSM-5 (Zeolite Socony Mobil #5), hexagonal mesosilicalite, nanotube halloysite, and Ag-silicalite, respectively. Drug release study indicates that Ag loading controls the Cpt release. The kinetics of drug release for the different formulations was examined using Korsmeyers-Peppas model. Release constant (k) and release exponent (n) were utilized for the rate of drug release and diffusion mechanisms, respectively. The in vitro study revealed an efficient interaction of nanoformulations promoting both antibacterial and anticancer activities.