Controlled Ruminal Infusion of Sodium Bicarbonate. 2. Effects of Dietary and Infused Buffer on Ruminal Milieu

Abstract

Four ruminally cannulated, lactating Holstein cows were assigned to a 4×4 Latin square to monitor the effects of NaHCO3 infusion on ruminal environment of cows receiving dietary sodium bicarbonate. Sodium bicarbonate (110g) was mixed with 3.8L of water and infused at a constant rate into the rumen from 0 to 2, 2 to 4, or 4 to 6h postfeeding twice daily. All cows were fed sorghum silage and concentrate in a 35:65 DM ratio for 45min twice daily. Ruminal fluid was collected at feeding and every 30min postfeeding for 8h on the last day of each 1-wk experimental period. Dry matter intake was not affected by NaHCO3 infusion. Yields of mik and its components were reduced with 4- to 6-h NaHC03 infusion. At certain isolated times, especially during infusion, NaHCO3 infusion increased ruminal fluid buffer. Concentrations of ruminal fluid total VFA were not affected by NaHCO3 infusions, whereas acetate to propionate ratio tended to be reduced. Ruminal liquid volume tended to be increased by 0- to 2-h NaHCO3 infusion, and ruminal outflow rate tended to be reduced by the 2- to 4-h infusion. Intraruminal infusion of NaHCO3 into cows receiving supplemental dietary NaHCO3 altered ruminal acid-base status as typically reported for those receiving dietary NaHCO3; however, these alterations were not accompanied by shifts in ruminal VFA patterns or in milk composition that normally result from such feeding regimens. The effects of NaHCO3 infused directly into the rumen may be different from those of dietary NaHCO3 and are possibly related to the different time of entry into the rumen relative to feeding.