Controlled-Release SHH Fibrin Scaffold Transplantation Promotes Function Recovery of the Spinal Cord

Abstract

Objective: investigate that the transplantation of sonic hedgehog(SHH) fibrin scaffold promotes recovery of the spinal cord injuryin rats. Method: first, the model of controlled-release SHH fibrinscaffold was made in vitro as the experimental group and observethe controlled-release performance. Second, 60 healthy SD ratswere assigned to prepare models of complete transection of spinalcord, divided into 3 groups: SCI group (simple transection ofspinal cord), FG group (fibrin group), F-SHH group (sonichedgehog-fibrin scaffold transplantation group). Grade hind limbs(BBB) of rats every week. The spinal cord segments were got outin 3 months after operation and went throughimmunohistochemistry and immunoblotting detection. Observe theexpression of NF200, GAP43 and GFAP. Result: (1) SHH Fibrinshowed a good effect of slow release. (2) F-SHH group showed amore significant improvement in BBB score that presented a risingtrend in the whole, compared with the other two groups and thedifference is statistically significant (P < 0.05). (3) The relativeamounts of NF200 and GAP43 in F-SHH group were much higherthan those in the other two groups, while the relative amount ofGFAP was lower and the difference is statistically significant (P <0.05). Conclusion: Controlled-release SHH fibrin scaffoldtransplantation will effectively recover complete spinal cordtransection of rats.