Abstract
Chitosan membranes loaded with bovine serum albumin (BSA; BSA/chitosan membranes) were prepared by either oven-drying or lyophilization. The encapsulation of BSA was mostly >80%. Controlled release of BSA from the membranes into phosphate-buffered saline (PBS; pH 7.4) was determined with Coomassie brilliant blue at different time periods. The highest cumulative amount of released BSA was 17.30% after 20 days. The optimized chitosan concentration was found to range from 10 to 20 g/l. The most favorable drying temperature for the membrane preparation was 55°C. And membranes made by lyophilization were more porous and uniform than those made by oven-drying.
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