Abstract
Abstract A novel tetracydine regulation expression system was used to regulate the expression of enhanced green fluorescent protein EGFP and hepatitis B virus precore protein in the mammalian cell lines with lipofectAMINE. Flow cytometry assays showed that application of the system resulted in about 18-fold induction of EGFP expression in CHO cell lines and 5-fold induction in SSMC-7721 cells and about 2-fold in the HEK293 cells. Furthermore the effective use of this system for the controlled expression of HBV precore protein gene in hepatocellular carcinoma cells was tested.
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