Controlled Delivery of Dopamine Hydrochloride using Surface Modified Carbon Dots for Neuro Diseases

Abstract

Delivery of therapeutic moieties using water soluble Carbon dots (C-dots) have been pivotal to control the release of the drugs under physiological condition due to their high biocompatibility. Controlled Dopamine hydrochloride (DA), a potential neurotransmitter using C-dots as carriers is studied in the present work, in order to highlight its potential to deliver drugs related with neurological disorders such as Alzheimer's and Parkinson's disease. The tenure of the DA release under the considered environment at pH 7.4 was extended to 60 h after conjugation to C-dots, in comparison to bare DA under the same environment. The DA release was as per Hixson-Crowell model. In order to understand the impact of the C-dots-DA conjugate under physiological conditions, Nero 2A cells were taken under consideration. The conjugate was found to have least impact as far as the toxicity of the nanoparticles is concerned. Microtomy of tissue section of vital organs was also performed to see the internal effect of conjugate drug. No inimical effect of the conjugate was found on internal organs of experimental models during histological studies. Less toxicity was found on Neuro 2A cells by C-dots-DA conjugate as compared to alone DA. Body weight of mice was also taken into consideration after injecting 20 µg mL−1 of C-dots and C-dots-DA, we did not found any change in their body weight till 45 days of observation.