Controllable olfactory cellular network formation on polyaniline conducting polymer modified microelectrode array

Abstract

Here a conducting polymer-based cell immobilization method was presented to form controllable olfactory cellular network on microelectrode array. PAIN conducting polymer was electropolymerized on the surface of microelectrode array in a sequence in order to modify the defined microelectrode. Different antibodies can be linked covalently onto defined sites for the effective capture and immobilization of different cells. Based on the specific recognition between antibody and cells, olfactory receptor cells were site-specifically immobilized on the surface of microelectrodes. The fluorescent staining results indicate that olfactory cells were captured effectively with the microelectrode array. Extracellular potential changes can be efficiently monitored from olfactory cells in response to odorant stimulations. All the results demonstrated that this immobilization method can be a universal method to control the arrangement of cells on the sensor surface, which have great application prospect in the field of cell-based biosensor.