Abstract
Abstract We report the effect of various parameters, namely substrate concentration, time, pH and temperature, on the biosynthesis of silver nanoparticles (AgNPs) by using the extract of actinobacterial strains, which were isolated from the sediments of Lonar Crater Lake in India. It was found that the formation of AgNPs and its morphology depended on synthesis conditions. Visual observation of the reaction mixture, ultraviolet-vis spectroscopic analysis and mass of synthesized AgNPs indicated that 25°C, pH 7 and 3 days of incubation time were optimal for its efficient synthesis. The transmission electron microscopy (TEM) analysis revealed aggregation and irregular shape of AgNPs both at acidic pH and below 25°C. It was found that alkaline pH and temperature higher than optimal fostered the formation of nanoparticle aggregates. Based on the obtained results, it was concluded that the efficiency of biological synthesis by using actinobacteria as well as the size and shape of fabricated nanoparticles can be manipulated by controlled conditions of synthesis process. The use of desired nanoparticles increases its potential for medical applications.
Highlights
Nanotechnology is a fast-growing branch of science that deals with the synthesis and development of different nanomaterials [1]
Four actinobacterial strains (OT1, OF1, OF2 and IF19) were isolated from the sediments of Lonar Crater located in Buldhana district, Maharashtra, India, by the dilution plate procedure described by Rathod et al [21] on the starch casein agar [22] supplemented with 5% (w/v) sodium chloride (NaCl), at pH 8.5
The present study demonstrated that the absorption intensity and amount of biosynthesized AgNPs increase along with the increase in pH value
Summary
Nanotechnology is a fast-growing branch of science that deals with the synthesis and development of different nanomaterials [1]. Silver nanoparticles (AgNPs) are the main focus of intensive study because of their wide applications, such as catalysts, optics, antimicrobials, anticancer agents, and for biomaterial production [1,2,3,4]. There is enormous interest in metal nanoparticles (NPs) because of their unique physical and chemical properties revealed at the nanoscale level [5]. The microbial recovery of noble metals with the formation of their NPs is a green alternative to physical and chemical methods, which use high amounts of energy or toxic solvents and reagents. A biological synthesis process provides a wide range of environmentally acceptable methodology, low-cost production and minimum time required [6]. The exploration of actinomycetes has recently gained interest for efficient biological synthesis of metallic NPs [6]
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