Abstract

This study was conducted in determine the efficiency of each of temperature, exposure time and bio-control agent Trichoderma harzianum in inhibition of the sclerotia myceliogenic germination of S. sclerotiorum and its mycelial growth on PDA and field. The laboratory experiment included determine efficient temperatures and its exposure hours needed to inhibit pathogen germination in order to use for minimizing disease dissemination in the field through soil solarization technique. The study showed that temperature of 60 ?C with exposure time of 5 and 6 hours were the most efficient treatments in inhibition of myceliogenic germination (55.55, 100 %) respectively. The result antagonism between T. harzianum and S. sclerotiorum by dual culture technique showed high efficacy of the bio-control agent T. harzianum inhibition anti mycelial growth of the pathogen S. sclerotiorum on PDA scoring antagonism rate of 2 degree. In terms of disease incidence the results showed that soil solarization alone and its integration with bio-control agent were completely prevented the occurrence of the disease achieving the maximum reduction in incidence rate (100 %) for all studied depths at the end of eighth weeks compared with control treatment in which the average of incidence was (85.42 %) , followed by the treatment of bio-control agent alone by a reduction rate of (42.08 %) respectively with a significant difference compared with inoculated control treatment.

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