Abstract
Regulation of gene expression within the bacteriophage λ genome is controlled, in part, by transcriptional termination and anti-termination mechanisms (i.e. attenuation). We define a discrete site, t R1, in the y region of λ at which transcription termination occurs in vivo. This termination site and the factors which regulate termination at this site in vivo are examined and compared with the previously described in vitro termination reaction. Mutations in the region that enhance ( cin-1) or reduce ( cnc) termination at t R1 in vitro appear to have similar effects in vivo. The cin-altered t R1 site appears to be partially resistant to the anti-termination effects of N function. In addition, genetic experiments with cnc mutants suggest the existence of additional termination sites in λ beyond t R1 but before t R2.
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