Abstract
Luminescent bacteria toxicity test has attracted considerable attention in the past ten years.However,maintaining the stabilization of the activity of luminescent bacteria is a demanding task,which seriously limits the application of such diagnostics in on-line determination and analysis.In this study,a control method of maintaining the stabilization of the intensities of Photobacterium phosphoreum and P.leiognathi was investigated by adding various cryoprotectants.In this method,the bacteria in the exponential growth phase was centrifuged,re-suspended in the cold skimmed milk solution and stored at 5 ℃.Three cold storage methods were studied and compared,and they were the skimmed milk-bacteria suspension,freeze-dried bacteria suspension made by rehydrating freeze-dried bacteria powder and fresh bacteria solution being stored at 5 ℃.The results indicated that the storage life using skimmed milk bacteria suspension could reach 7 days,with relative luminosity 93%.This method could significantly improve the stabilization of the luminescent bacteria,and it could be applied in on-line toxicity monitor to improve continuous operation time.
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