Abstract
The development of isocitrate lyase (threo-D S-isocitrate glyoxylate lyase, EC 4.1.3.1) (ICL) activity in cotyledons of germinating soybeans Glycine max (L.) Merr. is apparently the result of de novo synthesis, because cycloheximide completely inhibits the accumulation of enzyme activity. The mature soybean seed lacked ICL activity, and no activity was detected in the embroyonic axis at any stage of growth. Furthermore, the metabolism of the embryonic axis or developing seedling axis was not required for the accumulation of ICL activity in the cotyledons during germination and subsequent growth. The accumulation of ICL activity was not dependent on RNA synthesis, but did require further processing of the presumptive mRNA apparently present in the mature seed. The hormone, abscisic acid, completely inhibited the accumulation of isocitrate lyase activity and apparently required mRNA synthesis because actinomycin D and cordycepin, but not 5-fluorouracil, were effective in reversing the inhibition.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
