Abstract
Control of nonspecific/specific protein adsorption is the main goal in the design of novel biomaterials, implants, drug delivery systems, and sensors. The specific functionalization of biomaterials can be achieved by proper surface modification. One of the important strategies is covering the materials with functional coatings. Therefore, our work aimed to functionalize multilayer coating to control nonspecific/specific protein adsorption. The polyelectrolyte coating was formed using a layer-by-layer technique (LbL) with biocompatible polyelectrolytes poly-L-lysine hydrobromide (PLL) and poly-L-glutamic acid (PGA). Nonspecific protein adsorption was minimized/eliminated by pegylation of multilayer films, which was achieved by adsorption of pegylated polycations (PLL-g-PEG). The influence of poly (ethylene glycol) chain length on eliminating nonspecific protein adsorption was confirmed. Moreover, to achieve specific protein adsorption, the multilayer film was also functionalized by immobilization of antibodies via a streptavidin bridge. The functional coatings were tested, and the adsorption of the following proteins confirmed the ability to control nonspecific/specific adsorption: human serum albumin (HSA), fibrinogen (FIB), fetal bovine serum (FBS), carcinoembryonic antigen human (CEA) monitored by quartz crystal microbalance with dissipation (QCM-D). AFM imaging of unmodified and modified multilayer surfaces was also performed. Functional multilayer films are believed to have the potential as a novel platform for biotechnological applications, such as biosensors and nanocarriers for drug delivery systems.
Highlights
Control of specific/nonspecific protein adsorption is one of the main challenges in designing novel biomaterials, such as implants, sensors, or drug delivery systems
In our previous work, we were focused on the detailed investigation of multilayered polyaminoacids films modified with poly-L-glutamic acid (PGA)-g-polyethylene glycol (PEG), here, we focused on the fundamental study concerning the influence of PEG chain length of commercially available poly-L-lysine hydrobromide (PLL)-g-PEG on antiadhesive properties of such pegylated polyaminoacids multilayer coatings
We tested polyelectrolyte multilayer coating formed with biocompatible polyelectrolytes as functional coatings for controlling specific/nonspecific protein adsorption
Summary
Control of specific/nonspecific protein adsorption is one of the main challenges in designing novel biomaterials, such as implants, sensors, or drug delivery systems. It is expected that such biomaterials will likely be in direct contact with biofluids, such as blood or serum [1]. The presence of the components such as blood cells, lipoproteins, plasma proteins, peptides, and their nonspecific adsorption (called fouling or biofouling) is the most critical problem for practical applications of biomaterials besides cost considerations [2]. The novel biomaterials are very promising when tested in a simple, well-controlled environment; their effectiveness is not yet satisfied when tested in real-life environments with much more complex chemistries. The specific adsorption of proteins to biomaterials results mainly from noncovalent interactions between the system’s biomolecules
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
